Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.

نویسندگان

  • Mitri K Khoury
  • Ian Parker
  • Dana W Aswad
چکیده

We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A Semi-Automatic Image-Based Close Range 3D Modeling Pipeline Using a Multi-Camera Configuration

The generation of photo-realistic 3D models is an important task for digital recording of cultural heritage objects. This study proposes an image-based 3D modeling pipeline which takes advantage of a multi-camera configuration and multi-image matching technique that does not require any markers on or around the object. Multiple digital single lens reflex (DSLR) cameras are adopted and fixed wit...

متن کامل

An Integrated Sensor Orientation System for Airborne Photogrammetric Applications

There are now available excellent integrated sensor orientation systems for the direct measurement of sensor position and attitude using GPS and IMU technology. With the maturity of these systems has come a wider interest in using this technology in an everincreasing range of applications. With a variety of applications, there becomes a need for a variety of solutions, balancing quality and cos...

متن کامل

Sayisal Görüntü Anali̇z İşlemi̇nde Kamera Kali̇brasyon Parametreleri̇ni̇n Beli̇rlenmesi̇ Determination of Camera Calibration Parameters at Digital Image Analysis

It is possible to make measurements, to evaluate and form digital maps of the images by using digital image processing and analysis techniques with computers. Digital cameras are being used in computer vision applications commonly. Digital cameras have been absolutely calibrated to use precision need works. Photogrammetry is based on direct measurement of the objects which is captured with the ...

متن کامل

Combined Raman and FT-IR spectroscopy using the Illuminat/RTM module for Renishaw's inVia Raman microscopes - Application note

The IlluminatIR™ is commonly used with either a diamond contact ATR (attenuated total reflection) or a diffuse reflectance objective. The sample can be viewed, and Raman spectra collected, either through these objectives or through the high quality microscope objectives supplied with the inVia Raman microscope. Simple switching between video view, IR acquisition, and Raman acquisition allows IR...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Analytical biochemistry

دوره 397 1  شماره 

صفحات  -

تاریخ انتشار 2010